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Journal: Non-coding RNA Research
Article Title: Exosomal miRNA-218–5p derived from low-passage dermal papilla cells modulates hair follicle growth and development
doi: 10.1016/j.ncrna.2026.01.004
Figure Lengend Snippet: Exosomes derived from low-passage DPCs regulated HFSC proliferation. (A) Indirect immunofluorescence showing ALPL and PCNA expression in low-passage (P1) and high-passage (P8) DPCs (scale bar = 50 μm). (B) RT-qPCR analysis of HF development-related gene expression in P1 and P8 DPCs (unpaired two-tailed t -test, n = 3). (C) TEM images of exosomes from P1 DPCs (DPC-Exos P1) and P8 DPCs (DPC-Exos P8). (D) NTA measurement of particle size of DPC-Exos P1 and DPC-Exos P8. (E) Western blot detection of exosome-specific proteins in DPC-Exos P1 and DPC-Exos P8. (F) CCK-8 assay evaluating HFSC proliferation after treatment with DPC-Exos from P1 and P8 (one-way ANOVA, n = 5). (G) Flow cytometry analysis of HFSC apoptosis after treatment with DPC-Exos from P1 and P8 (one-way ANOVA, n = 3). ∗ P < 0.05, ∗∗ P < 0.01.
Article Snippet: The
Techniques: Derivative Assay, Immunofluorescence, Expressing, Quantitative RT-PCR, Gene Expression, Two Tailed Test, Western Blot, CCK-8 Assay, Flow Cytometry
Journal: Bioactive Materials
Article Title: Food-derived β-lactoglobulin nanofibrils: An efficacy, safe, and scalable solution to overcome oral insulin delivery challenges
doi: 10.1016/j.bioactmat.2025.11.020
Figure Lengend Snippet: Safety evaluation of orally administered nanofibrils. (a – b) Biodistribution analysis: (a) In vivo fluorescence imaging and (b) ex vivo organ distribution of Cy5.5-labeled nanofibrils and monomers (n = 3). (c) mRNA levels of inflammatory markers in the colon (n = 6). (d) Histopathological evaluation of intestinal tissues by H&E staining (n = 3). (e – f) Apoptosis assessment: (e) Representative TUNEL staining images and (f) quantitative analysis revealing comparable apoptotic cell counts across groups (n = 3). (g – k) Gut microbiota profiling following 21-day oral administration (n = 6): (g) Relative abundance of microbial taxa at genus level; (h) Alpha diversity analysis; (i) Beta diversity analysis; (j) LEfSe analysis; (k) KEGG pathway enrichment analysis.
Article Snippet: Tissue sections were fixed in paraformaldehyde, and apoptosis detection was performed using the
Techniques: In Vivo, Fluorescence, Imaging, Ex Vivo, Labeling, Staining, TUNEL Assay